Techniques For Invitro Pharmacology Lab Report Biology Essay

Techniques For Invitro Pharmacology Lab Report Biology Essay Schild plot: Schild plot is defined as pharmacological method of receptor classification. By using schild plot dose-effect curve for an agonist is determined in the presence of various concentrations of a competitive antagonist for its receptor in the presence of agonist i.e. equilibrium dissociation constant is calculated. The experiment is carried out for series of dose ratios for a given effect. For example the ratio of the dose of agonist (A) to produce a specific effect (e.g.,  half maximal effect) in the presence of the antagonist (B) to the dose required in the absence of the antagonist (A) is calculated. This is determined for several doses of antagonist and then log ((A/A) -1) versus the negative log B is plotted.   If the regression of log ((A/A) -1) on -log B is linear with a slope of -1, then this indicates that the antagonism is competitive and by definition the agonist and antagonist act at the same recognition sites. If the slope of the regression is not -1, then b y definition the antagonist is not competitive or some other condition is in effect. This might include multiple binding sites or pharmacokinetic interactions. Agonist: Agonist is a drug which has both affinity and efficacy. Antagonist: Antagonist is a drug which has affinity and zero efficacy. Affinity:  Affinity is a property of a drug; it measures how tight a drug binds to a receptor. To bind to a receptor a functional group of the drug should bind to the complementary receptor. The binding capacity of the drug defines the action of the drug. Efficacy: Efficacy of a drug can be defined as ability of drug which activates the receptor to produce desired effect after binding. Affinity and efficacy are explained in the equation as: K+1 ÃŽ ± A + R AR* Response K-1 ÃŽ ² K+1 B + R BR No Response K-1 Where A is agonist, B is antagonist, K+1 is association rate constant for binding, K-1is dissociation rate constant for binding ÃŽ ±- Association rate constant for activation ÃŽ ²- Dissociation rate constant for activation By using law of mass action affinity is explained as B + R BR Drug free receptor drug-receptor complex At equilibrium KB = [R] [B] KB = Equilibrium dissociation constant [BR] Hill-Langmuir equation: this equation explains drug occupancy [RT] = [R] + [BR] If [RT] = Total number of receptors then by substituting this in law of mass action equation [RB] = [B] [RT] KB + [B] By this equation it is determined that drug occupancy (affinity) depends on drug concentration and equilibrium dissociation constant. Equilibrium dissosciation constant: EQUILIBRIUM DISSOCIATION CONSTANT (Kd) : It is the characteristic property of the drug and the receptors. It is defined as the concentration of the drug required to occupy 50 % of the receptors. The higher the affinity of the drug for the receptors lower is the Kd value. Mathematically Kd is k2/k1 where k2 is the rate of dissociation of the drug from the receptor and k1 is the rate of association of the drug for the receptor. Receptor (R) and Drug (D) interact in a reversible manner to form a drug-receptor (RD) complex.   Ã‚  Ã‚  Ã‚  Ã‚  Where R =   Receptor   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  D =   Drug (L for ligand is sometimes used in these equations)   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  k1 = the association rate constant and has the units of M-1min-1   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  k2 = the dissociation rate constant and has the units of min-1.     Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  k2 is sometimes written as k-1. If an agonist binds to the receptor, then the interaction of the agonist (D) and the receptor (R) results in a conformational change in the receptor leading to a response. If an antagonist binds to the receptor, then the interaction of antagonist (D) and receptor (R) does not result in the appropriate conformation change in the receptor and a response does not occur. For drugs that follow the law of simple mass action the rate of formation of the complex can be defined by the following equation d[RD]/dt refers to the change in the concentration of [RD] with time (t). Note: the square brackets refer to concentration. This equation indicates that the rate at which the drug receptor complex (RD) is formed is proportional to the concentration of both free receptor (R) and free drug (D). The proportionality constant is k1. The rate of dissociation can be defined by the following equation -d[RD]/dt is the decrease in drug-receptor complex with time This equation indicates that the rate at which the drug-receptor complex (RD) dissociates back to free drug and free receptor is proportional to the concentration of the drug receptor complex. The proportionality constant is k2. When the drug and the receptor are initially mixed together, the amount of drug-receptor complex formed will exceed the dissociation of the drug-receptor complex. If the reaction is allowed to go for a long enough, the amount of drug-receptor complex formed per unit time will be equal to the number of dissociations of drug-receptor complex per unit of time, and the system will be at equilibrium. That is equilibrium has occurred.    Equilibrium can be defined as or k1[R][D] = k2[RD] This equation can be rearranged to give By definition Kd is the dissociation equilibrium constant. Kd has units of concentration as shown in the following equation. Simple competitive antagonism: simple competitive antagonism is the most important type of the antagonism. In this type of antagonism the antagonist will compete with available agonist for same receptor site. Sufficient antagonist will displace agonist resulting in lower frequency of receptor activation. Presence of antagonist shifts agonist log dose response curve to right. A schild plot for a competitive antagonist will have a slope equal to 1 and the X-intercept and Y-intercept will each equal the  dissociation constant  of the antagonist. This can be explained in equation as: Occupancy for agonist [RA] = [A] OR [A]/ KA [RT] KA+ [A] [A]/ KA +1 In presence of competitive antagonist (B) [RA] = [A]/ KA [RT] [A]/ KA + [B]/ KB + 1 Occupancy reduced according to [B] and KB To obtain same occupancy, must increase [A] to [A`] r = [A] / [A] = [B] / [B] Schild equation: r = [B] / KB +1 Where r depends on [B] and KB Applying log on both sides log (r-1) = log[B] log KB Aim: The main aim of the experiment is to measure the equilibrium dissociation constant (KB) for atropine at acetylcholine muscuranic receptors and to determine the drug receptor interactions. Objectives The main objectives of the experiment are as follows To measure the equilibrium dissociation constant for atropine at acetylcholine muscuranic receptors To demonstrate the reversible competitive antagonism of atropine at acetylcholine muscuranic receptors To determine the equilibrium dissociation constant (KB) for atropine at acetylcholine muscuranic receptors by using schild plot. Method Isolation and mounting of Guinea-pig ileum in organ bath Guinea-pig was first sacrificed and then the ileum was collected and transferred into physiological salt solution maintained at 370C. The food particles present in the ileum was expelled out through running Krebs solution through the lumen. Then tissue was tied with a thread at both the ends where one was tied to the mounting hook and the other was attached to the transducer. Preparation of serial dilutions of drug The drugs used in the experiment were acetylcholine (Ach) and atropine. To determine the simple competitive antagonism of atropine at Ach muscuranic receptors serial dilutions of Ach were carried out. Ach was given as 110-2M and from the above concentration of the drug the following concentrations were prepared to the organ bath concentration such as 110-6M, 310-6M, 110-7M, 310-7M, 110-8M, 310-8M, 110-9M and 310-9M Ach. Then atropine was diluted to 110-8M (organ bath) from the given 110-2M concentration. Determination of Organ bath concentration The volume of physiological salt solution (pss) was 20 ml, and each time the volume of drug introduced into organ bath was 20 µl.Therefore if 20 µl of 110-2M drug was introduced into the organ bath then it gives 110-5M organ bath concentration. Mathematical calculation of organ bath concentration: In organ bath we have 20ml of pss which is equal to 20103  µl of pss, if 20  µl of 110-2 M Ach was introduced then the organ bath concentration 20 µl→XM 20ml→10-2M = 20  µl x 10-2 M 20x 103  µl = 110-5M (organ bath concentration). The isolated guinea- pig ileum was mounted onto the organ bath and set up for recording isometric tension of the tissue using chart software in a Mac book. Step-1 Calibration of the experimental apparatus: The chart 5 software was calibrated and the sampling rate was adjusted to 10 samples per second with a maximum input voltage to 10 mV. The baseline was set to zero and then trace was started from the baseline zero then the force transducer was calibrated by placing 1 gram weight and after the calibration the trace produced was stopped for the moment to convert the units of tension into grams by selecting the trace produced previously. Step-2 Sensitisation of preparation: To check the viability of the tissue a response of suitable height was obtained by adding a little high concentration of the drug. Here in the experiment an appreciable recording was noted at 110-7M Ach. Step-3 The time cycle followed to construct a concentration- response curve was 0 seconds to add the drug concentrations 30 seconds to empty the organ bath and refill with fresh physiological salt solution 180 seconds next drug concentration was added to the organ bath. Concentration Response Curve: By making use of the above drug concentrations a concentration response curve was constructed according to the provided time cycle. 20  µl of 110-9M Ach was added into the organ bath at zero seconds at is allowed to stand for 30 seconds, then after 30 seconds the organ bath was emptied and refilled with pss. Pss was allowed to stand for 180 seconds. During the wash period if the peak does not return to the base then it was washed twice or thrice to make sure that all the drug dissociates from the receptors before the next addition of the other drug concentration. Each concentration was repeated twice or thrice until the two consecutive responses were reported with the same peak height. By following the procedure and time cycle, the concentration response curve was constructed with different concentrations of acetyl choline such as 110-9M,310-9M, 110-8M, 310-8M, 110-7M, 310-7M, 110-6M and 310-6M Ach (organ bath concentration). Step-4 Equilibration of Acetylcholine receptors with acetylcholine After step-2 the preparation was washed several times until the peak returned to the base line. Then atropine (110-8M organ bath concentration) was added to the preparation and then set aside for 40 minutes to allow atropine to equilibrate with acetylcholine muscuranic receptors. Step-5 Concentration response curve in the presence of atropine The concentration response curve with acetylcholine was repeated again in the presence of atropine by following the time cycle and procedure, which was same as same step 2.Therefore in step 3 with each addition of acetylcholine concentration atropine was added simultaneously. Step-6 Analysis: The graph pad prism in the Mac book was used to plot concentration response curves in the absence and presence of atropine. Log concentration (acetylcholine) Vs response in grams From the above plot EC 50 values of acetylcholine in the presence and absence of atropine were obtained. Then the distance between the two curves control and response for the atropine presence was denoted by r, where r was called as shift. The shift was calculated mathematically as r= EC 50 of response in the presence of atropine EC 50 of Ach in the absence of atropine From the value of the shift, schild plot was plotted as log concentration of atropine presence against log(r-1). From the schild plot the dissociation constant KB for atropine at acetylcholine muscuranic receptors was determined. Results: As explained above in the procedure serial dilutions of acetylcholine was added to the organ bath, where Ach has produced concentration dependent contractions of the guinea pig ileum as shown in the fig 1. Figure: 1 Trace showing contractions produced by serial dilutions of acetylcholine at muscuranic receptors. As shown in Figure 1 the serial dilutions of acetylcholine are added into the organ bath from 110-7M to 310-6M Ach. Here in the trace it was clearly shown that contractions produced by the acetylcholine have been increased with respect to the concentrations. In step-2 the preparation was washed and added with 110-8M atropine and set aside for 40 minutes to equilibrate the acetylcholine receptors. Figure: 2 Trace showing contractions produced by serial dilutions of acetylcholine at muscuranic receptors in the presence of atropine. In the trace it is clearly shown that, the contractions produced by serial dilutions of Ach from 110-8M to 310-4M in the presence of 110-8M atropine. When Trace 1 and Trace 2 are compared it is evident that the contractions produced by Ach alone (trace 1) were greater than the contractions produced Ach in the presence of atropine (trace 2) which proves the simple competitive antagonism by atropine at muscuranic receptors. A graph is plotted to the log concentration response curve produced by Ach alone against Ach in the presence of atropine. (graph is attatched to the report) From the graph it is known that with the increase in the concentration of Ach, response have been increased when compared to Ach in the presence of atropine and also there is a shift towards right which shows the simple competitive antagonism produced by atropine. From the results produced by Ach alone against Ach in the presence of atropine the fractional difference which is called as shift can be obtained as follows Mathematical Calculation shift r = EC50 of response after atropine (or) in the presence of atropine EC50 of control (or) Ach in the absence of atropine = 2.5110-6 = 8.36 3.0 x10-7 r-1 =8.36 -1=7.36 log(r-1)=log (7.36) =0.86 Partial dissociation constant (PKB) or PA2 is measured to confirm the simple competitive antagonism, where pKB values play an important role in classifying receptors. Therefore PKB =log(r-1) -log [atropine] =0.86 -log (110-8) =0.86 (-8) =0.86+ 8 =8.86 From the above results log EC50 values for control (Ach alone) and Ach in the presence of atropine were given as 3.0e-007 and 2.51e-006 respectively. This shows the molar concentration of Ach which produces 50% of the maximal possible response is higher than the molar concentration response produced by Ach in the presence of atropine. Figure 5: (Graph2) Schild plot If the antagonist is competitive, the dose ratio equals one plus the ratio of the concentration of antagonist divided by its Kd for the receptor. (The dissociation constant of the antagonist is sometimes called Kb and sometimes called Kd) A simple rearrangement gives: Here we have plotted a graph with log (antagonist) on the X-axis and log (dose ratio -1) on the Y-axis. If the antagonist has shown simple competitive antagonism then the slope should be 1.0, X-intercept and Y-intercept values should be both equal the Kd of the antagonist obtained. If the agonist and antagonist are competitive, the Schild plot will have a slope of 1.0 and the X intercept will equal the logarithm of the Kd of the antagonist. If the X-axis of a Schild plot is plotted as log(molar), then minus one times the intercept is called the pA2 (p for logarithm, like pH; A for antagonist; 2 for the dose ratio when the concentration of antagonist equals the pA2). The pA2 (derived from functional experiments) will equal the Kd from binding experiments if antagonist and agonist compete for binding to a single class of receptor sites. Figure 6: (table 2) Results for Schild Plot. From Figure 5 and 6 it is evident that no concentrations of atropine have showed competitive antagonism perfectly. Therefore from the above results it is known that the concentrations of atropine has not shown simple competitive antagonism fairly. Discussion: Reversible competitive antagonism: The binding of drug to a receptor is fully reversible which produces a parallel shift of the dose response curve to the right in the presence of an antagonist. The mechanism of action of acetylcholine at muscuranic receptors: In various gastrointestinal smooth muscles, acetylcholine and its derivatives produce contractions by activating muscuranic receptors. It is generally assumed that the M3 muscuranic receptor plays a key role in mediating this activity. The M3 receptor is coupled preferentially to Gq-type G proteins, resulting in the activation of phospholipase C (PLC) and the formation of ionositiol trisphosphate (IP3) and diacylglycerol (DAG) which are likely to participate in muscuranic receptor-mediated smooth muscle contractions. IP3 causes Ca2+ release from intracellular store and can also mobilize Ca2+ secondarily through Ca2+-sensitive or store-dependent mechanisms. DAG, via activation of protein kinase C, phosphorylates various proteins and can directly activate non selective cationic channels. Figure 7: Diagrammatic representation of calcium and smooth muscle contraction. From the above results the value of shift obtained was 0.378 which denotes the simple competitive antagonism produced by the concentration of atropine used (110-8 M).From the value of shift the pKB value was calculated as 8.4.If atropine has shown simple competitive antagonism then the value of pKB should be equal to 1-X intercept. Therefore pKB=1-X intercept =1-(-8.86) =9.86 We got value of pKB as 8.86.Therefore pKB is not equal to 1-X intercept. Therefore the concentration of atropine (110-8M organ bath concentration) used by our group has not shown simple competitive antagonism effectively. The literature value of pKB is given as approximately 9 and we have obtained the value of pKB as 8.86 which does not fit with literature value. Therefore from the above observations and results i can conclude that a little more high concentration of atropine may serve to produce complete simple competitive antagonism by atropine at acetylcholine muscuranic receptors.

Hamlet is a thinker not a man of action Essay

Hamlet’s one mission in the play is to revenge the death of his father by killing Claudius, however his procrastination leads to his untimely death, the deaths of many others in the Danish court and the relinquishment of Denmark to Fortinbras. Hamlet’s first words show a desire of revenge towards Claudius â€Å"A little more than kin and less than kind. † But later in his soliloquy we see that he is actually closer to killing himself, than killing Claudius or the perpetrator: â€Å"O that this too too sullied flesh would melt, / Thaw and resolve itself into a dew,† He doesn’t even contemplate killing Claudius; he hopes that the situation will resolve itself, which it never will, showing his unwillingness to act. This soliloquy also shows that he is not the bravest of people, as he cannot tell his mother how he really feels, another restrictive character trait when trying to revenge someone: â€Å"But break, my heart for I must hold my tongue. † In his soliloquy straight after Hamlet’s conversation with the Ghost he seems determine to kill his uncle, â€Å"thy commandment alone shall live / Within the book and volume of my brain. † The use of the word â€Å"commandment† shows that he will follow the Ghost’s word religiously. This shows that he is resolute, as a man of action would be. However, this is countered almost immediately at the end of the scene â€Å"O cursed spite, / That ever I was born to set it right. † This shows that Hamlet is scared to carry out what he has to do, he would much rather someone else revenge his father than he. The first device that Hamlet uses to carry out his revenge is to pretend to be mad. By this pretence he hopes to draw the attention away of the court away from him so that he can watch and follow Claudius to see if he is showing any signs of guilt. He tells Guildenstern of his madness. â€Å"I am mad but north-north-west. When the wind is / southerly, I know a hawk from a handsaw. † His stated intention is to gain irrefutable evidence of Claudius’s villainy. He initiates this by visiting Ophelia in a state of undress and handing her a love letter, making Polonius believe that his madness is due to Ophelia’s rejection of him. Hamlet uses his feigned madness to show his true emotions and insult people he doesn’t like: â€Å"You are a fishmonger. † A fishmonger in Elizabethan times could have meant pimp, showing that Hamlet thinks that Polonius is using his daughter to gain favour within the court. Hamlet’s feigned madness does affect Claudius: in the first act he delivers long speeches, but by Act 2 he is reduced to short sentences like â€Å"We will try it. † This is an action, but it is one that allows him to procrastinate. When Hamlet is left alone he laments his weakness and inactivity. An actor could weep at the imagined grief of Hecuba, whereas Hamlet fails to respond to the murder of his father: â€Å"Tears in his eyes, distraction in his aspect, / A broken voice, and his whole function suiting / forms to his conceit? And all for nothing! / For Hecuba! † Hamlet’s principles cause him a great deal of self-criticism: â€Å"Why what an ass am I! This is most brave, / That I the son of a dear father murder’d, / Prompted to my revenge by heaven and hell, / Must like a whore unpack my heart with words/ And fall a-cursing like a very drab,† He curses his inactivity, showing that, although he is a thinker, he would prefer to be a man of action. At the end of the soliloquy he seems resolved to revenge Claudius, the â€Å"Mouse-Trap† play is a form of revenge against Claudius, but again it is not direct revenge, as he is still procrastinating. The fact that he has not confronted Claudius four months after confirmation from the ghost that Claudius is the guilty party shows that he is definitely a thinker. Hamlet’s soliloquy at the start of Act 3 still shows his overwhelming desire to think, particularly about suicide â€Å"To be or not to be†. The fact that he is still has time for soliloquies, and that he is not trying to hunt Claudius down and kill him, shows that he is definitely a thinker. Hamlet shows a lack of self knowledge as he cannot, as he intended in Act 1 sc 5 â€Å"with wings as swift / As meditation or the thoughts of love / May sweep to my revenge. † Instead he broods on his father’s death and even when he gets proof from Claudius’s reaction to the Mouse Trap play, â€Å"I’ll take the ghost’s word for a thousand pound. † He hesitates and needs further spurring by the ghost in Act 3 â€Å"to whet thy almost blunted purpose. † Hamlet has the perfect opportunity to kill Claudius in Act 3 sc 3, but again he procrastinates, letting himself think about what will happen to Claudius’ soul â€Å"A villain kills my father, and for that I, his sole son, do this same villain send to heaven. † He doesn’t act, because he thinks that Claudius is praying, cleansing his soul. This would send him to heaven, not hell where he belongs. The irony is that Claudius himself has too much on his conscience and cannot pray, â€Å"My words fly up, my thoughts remain below. / Words without thoughts never to heaven go†. Hamlet curses himself in a later soliloquy for his lack of action. Hamlet’s first action of physical revenge is to stab Polonius behind the arras. This action shows that he can only do something on the spur of the moment. If he’d had time to think about it, he would have found a way around stabbing the person behind the arras, electing instead to procrastinate. Hamlet meets Fortinbras’ army in Act 4 sc 4, which makes him feel depressed when he compares himself to Fortinbras: the Norwegian Prince is prepared to fight over something of very little value, while he hasn’t yet taken revenge for the murder of his father and the seduction of his mother: â€Å"How all occasions do inform against me, / And spur my dull revenge. † Hamlet does what he always does when confronted with a problem; he has a soliloquy. However, this is his last soliloquy, which could suggest that he is done with thinking now, and will finally carry out his revenge. Hamlet shows another decisive action, in dealing with the betrayal of his one-time friends, Rosencrantz and Guildenstern and with Claudius’ attempt to have him killed on his way to England. He replaces his own name with that of Rosencrantz and Guildenstern in the letter, ensuring that they will be killed in his place. He also Boards a pirate ship so that he can return to Denmark, these are all very decisive actions focused to towards revenging his father’s death. Hamlet’s entrance into Ophelia’s funeral certainly is more action-focussed than his previous actions: â€Å"This is I, / Hamlet the Dane. † His fight with Laertes, declaration of his love of Ophelia and his switch from prose to verse show that he is longer talking his self into lying and misleading others. Therefore he is not thinking as much as he is acting. Hamlet’s next action is to duel with Laertes, not knowing that it has been fixed so that Hamlet will die, but as with most strategies in the play, it does go according to plan: Both Laertes and Hamlet are wounded by the poisoned sword, Gertrude drinks the poisoned wine, and one of Hamlet’s dying acts is to force Claudius to drink the poisoned wine, which he does with relish, enjoying the power he has, and the fact that he is killing the person who killed his father, seduced his mother, taken his thrown and plotted to kill him twice’ â€Å"Here thou incestuous, murd’rous, damned Dane, / Drink off this potion. Is thy union here? / Follow my mother. † His action here was done purely, without any thought. As he dies Hamlet names Fortinbras as his successor to the throne of Denmark. He admires Fortinbras as a man of action, seeing that that is what his country needs to return stability to it, â€Å"I do prophesy th’election lights / On Fortinbras. He has my dying voice. † The time frame of the play helps to reinforce the impression of time passing. Individuals in the play travel from Denmark to Norway, Poland and England, from the court to the countryside. Contrasting the activity of Laertes and Fortinbras with the prolonged inactivity of Hamlet. As the hero in this tragedy Hamlet doesn’t have one, sole, character flaw that leads to his untimely death. He is a thinker involved in a dilemma that can only be solved successfully by a man of action. His inability to act swiftly and decisively without high motivation in connection with his father’s murder brings havoc to the Danish court, his own death and the death of many others in the court. If he had been a man of action Claudius would have been killed months before.

Cookies Filled with Ice Cream Feasibility Study

Food is very essential for us. It is our need in order for us to live. There are many kinds of food that best suit on our taste buds. One of it is cookies. Cookies is the type of food that most of all like to eat because of its taste. Cookies is the kind of food that kids are attracted to eat. What with cookies that many, like to eat? We as student researchers delved into topic to find out what are the advantages of cookies that is filled with ice cream specifically in Bacolod City. Our study focused on advantages of cookies filled with ice cream. We choose Bacolod City people as our participants because related indication shows that many business creations are in the place that most people are present. We will present this research paper in order for us to know the taste and effect of cookies that is filled with ice cream in the consumption of people, reasons for eating, effects and the overall impact of eating cookies filled with ice cream in their daily lives. Our findings will be beneficial in both school and establishments’ in better understanding the advantages of cookies filled with ice cream. And we also offers different flavors of ice cream such as vanilla, mango, chocolate, and ube. Selecta is our choice from where we should get our ice cream to pair with the cookies. Significance of the Study: We conducted this research to find out the consumers’ reaction in the cookies filled with ice cream and the impact of it to different status quo. This will benefit various businesses and establishments on finding out the trend of consumers to better improve and innovate their products with an increase in profit. Our study can also help establishments that sell cookies to improve their relationship with their customers by satisfying them with irresistible taste and worthy service. Scope and Limitation: 1. Area of locality- Our study will focus on areas in the vicinity of Bacolod city 2. Subject or population- subjects will consist of people within Bacolod City. 3. Duration or Period- the study will be conducted in the months of August to October 2013. Logo: Chapter 2 Management Aspect Study Objectives: The primary reason for this study is to know weather the cookies filled with ice cream will hit the market. This is also to make a twist between eating a cookies with ice cream. This is also a solution for the hot weather here in the Philippines. Form of Business Organization: This busines is a PARTNERSHIP BUSINESS, consist of 6 partners. These 6 person will help each other to make their customers happy. Rules and Regulations: a. To be truthful and honest in all his/her endeavors particularly in his/her dealings. with a customer and to the business partners. b. To strictly observe all the rules and regulations enforced by the partners and not to violate them. c. To refrain from directly or indirectly misleading customers or verbally or nonverbally misrepresenting the company. d. To properly behave at all times and not to commit any act which may put his/her dignity and the business reputation. Duties and Responsibilities: a. All the 6 membersof this business is not only the owners of the business. They must also act as an employee and serve the guest well. b. All employess should maintain cleanliness within the area for food sanitation and safety. c. Owners and employees should treat each other regardless of what position the other one has to maintain peace and harmony in the company. Chapter 3 Product Description: Our signature cookies filled with ice cream is specially made for those who wants to eat cookies and ice cream at the same time. All of our products include a complimentary note card with your personal message whether it's for your parents, friend or even your partner in life. Production Process: First, all the ingredients are fed into a large batch tank. Instead of eggs and milk, dry egg powder and dry milk are most likely used. After everything is well-mixed, the dough is transferred to a machine with two feed rollers. The feed rollers turn to pull the dough into the machine and push it out through several openings of the proper shape/size (in this case likely a 2 inch diameter circle). As the product is going through the opening, a wire comes across and slices the cookie pieces off so that they land on a tray. The trays are then transferred to a large oven and baked in batches. Alternatively, the cookie pieces might transfer onto metal trays and travel through an oven continuously. After a cooling stage, the cookies are ready to serve with ice cream.

The Unseen Crimes Of Domestic Violence - 2302 Words

Adam Green Mrs. Barnes English IV February 25, 2015 The Unseen Crimes Serial killers, hitmen, premeditated murderers, there are all things that are in society that are known and feared. However these violent acts go unnoticed within the most secure unit of our everyday lives, our homes. Domestic violence is a problem that has been spread worldwide. It is also unfortunately a problem that dates time itself. Domestic violence is violence that is expressed toward loved ones. Domestic violence can range from pinching or pushing all the way down to choking, stabbing, shooting, and murder. Not only is domestic violence physical abuse toward a partner, it can also be an emotional abuse toward them as well. For instance, stalking is considered repeated harassment, and can also be considered threatening toward an individual. While most of the time domestic violence is carried out by men, women are not left out of the equation of this dreadful crime. People often can see the signs and refuse to address it. They may even think that it is none of their business. Most of the time, the abused partner will never come out and tell someone that they are being abused for fear of what the abuser might do to them. The only way that things can get better is for someone to take notice, be there for the abused, and to notify the proper authorities of the acts of abuse that is going on within that home. They can take care of the problem the way it is supposed to be taken care of. Domestic violenceShow MoreRelatedIts Time to Speak Out Against Domestic Violence Essay561 Words   |  3 Pageshusband or boyfriend. In this country, domestic violence is almost as common as giving birth. There is, however, hope for these women; help is available. nbsp;nbsp;nbsp;nbsp;nbsp;Domestic violence occurs way too often in American society. There are many reasons why people need to speak out against domestic violence. One reason is that statistics show that most abusers will continue to abuse until they are arrested. Another reason is that domestic violence doesnt only physically harm a woman;Read MoreDomestic Violence, A Federal Crime Essay1530 Words   |  7 PagesAn Unseen Crime A crime that affects between one and four million women in the U.S yearly is domestic violence. This crime, which many do not know is a federal crime, responsible for about thirty percent of female murders (Asher, Elba, Sugg 1). According to the American Bar Association (ABA), 90-95% of domestic violence victims are women (2) and â€Å" 70% of intimate homicides are female† (2) intimate murder, as opposed to murder by a stranger. Women today need to understand what domestic violence isRead MoreDomestic Violence Among Black And Blue1921 Words   |  8 Pagesthan on the bruises that cover her body.   The sponsors of the public service announcement put a gold and white dress on a battered woman to incorporate a popular phenomenon with something serious: domestic violence.  Within the last decade,  there has been an increase  in reported incidents  of domestic violence  among upper-class renowned  Americans males.     Martha Ross, a reporter,  interviewed a  few  of these men.   All of them blamed their actions on â€Å"accidents†Ã‚  or  the woman (Charlie Sheen?).  Ã‚  DespiteRead MoreProtective Legislations For Women s India2272 Words   |  10 Pagesconstitution, but the violence against women in the private domain remained unseen in the legal provisions of the country. Many movements took place to make the violence against women in the private domain to get legal sanction. Anyways, women get much legislation under the Indian constitution for their protection yet none of these legislations have clearly considered domestic violence as a crime till 2005. The failure of the existing provisions to address the domestic violence as a crime has led to theRead MorePost Traumatic Stress Disorder ( Ptsd )1246 Words   |  5 Pagessuffers from post-traumatic stress disorder can have symptoms such as uncontrollable thoughts about the event, severe anxiety, nightmares, and flashbacks. This means that the person with PTSD may b e involved in an incident where they may commit a crime unexpectedly because of a certain flashback they might have had at a specific moment and time. Sometimes it can be a simple situation where he or she may be forgiven due to the fact that they have a mental condition but this is not always the caseRead MoreIndian Removal Act Of The Early Nineteenth Century2595 Words   |  11 PagesStates government should do more to help Indian casinos, domestic violence cases, and poverty and land disputes because of the sovereign nation’s troubles with these issues. The opposition to the government being more of a factor in the Native American activities would argue that the United States were the ones that put them in this position in the first place. They are the reason that all of the substance abuse, poverty, domestic violence, and land disputes have occurred on the reservations so farRead MoreThe Effects Of Long Term Alcohol Use On The Brain1482 Words   |  6 Pagesirresponsibility and domestic violence, proving that the consequences of alcohol use affect more than just the user. Alcohol is often a common ground in cases of violence, public disturbances, and incidents of self-harm. Families torn apart by alcohol struggle to maintain composure; fortunately, there are support groups such as â€Å"Al-Anon† that offer aid to people affected by an alcoholic loved one. Alcoholism also can cause a strange, polar-opposite consequence in family members. The unseen impact of alcoholismRead MoreHuman Dignity And The Inalienable Rights Of Ever y Person1251 Words   |  6 Pagesbasic features of enslavement. The smuggling of immigrants is the act of helping citizens to leave a country illegally or residing illegally in another country with the intention of having benefits for the movement a style of profitable business. This crime is often organized by criminal networks, which take hold of the occasion to make large proceeds from an unlawful movement without having to worry about authorities. While there are significant differences between trafficking persons and human smugglingRead MoreThe Effects Of Sleeplessness And Its Effects On Children Essay1447 Words   |  6 Pageshe reseved after losing his loved one was too great, he had kept himself awake reading old horror stories and still he hopes the now dead Lenore would come back, which was inhumanely impossible, â€Å"Then methought the air grew denser, perfumed from an unseen censer Swung by Seraphim whose footfalls tinkled on the tufted floor. Wretch, I cried, thy God hath lent thee—by these angels he hath sent thee Respite—respite and nepenthe, from thy memories of Lenore! Quaff, oh quaff this kind nepenthe and forgetRead MoreThe Truth About Human Trafficking1520 Words   |  7 Pagesaround the world and it was not until 1995, â€Å"the United Nations held the fourth World Conference to address the issue of trafficking of women. In this meeting, a major accomplishment was the fact that trafficking was actually recognized as an act of violence against women, and the concept of trafficking was further defined†(Timeline of Human Trafficking). The Polaris project was founded in 2002, which is an organization that has the aim of creating a society that does not contain human trafficking (The